Occurrence of nucleic acid hydrolyzing IgM antibodies in rheumatoid arthritis patients’ sera
Abstract
Objective: Study goal was to investigate the nuclease like catalysis function of polyclonal IgM Abs recovered form rheumatoid arthritis (RA) patient sera.
Methods: Intact polyclonal IgM Abs was recovered from the RA patient in a single step using CIM® EDA monolith column. The DNA hydrolysis and RNA assays, in-situ and thermal inactivation, were performed to examine and measure the catalysis activity.
Results: The RA IgM Abs exhibited moderate hydrolysis of plasmid DNA, pUC18 and total yeast RNA, respectively. Non-negligible differences in the nuclease activities were prominent among the patient’s samples, in spite of an overall moderate activity. Result of RNA in-situ assay revealed that the catalysis function is mediated by µ chain, thereby confirming it to be an intrinsic property of IgM Abs.
Conclusion: The current preliminary study has concluded that IgM Abs from RA disease possess nuclease activity and IgMs mediate hydrolysis of both DNA and RNA macromolecules.
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